Classification and Uncertainty Visualization of Dendritic Spines from Optical Microscopy Imaging

Abstract

Abstract Neuronal dendrites and their spines affect the connectivity of neural networks, and play a significant role in many neurological conditions. Neuronal function is observed to be closely correlated with the appearance, disappearance and morphology of the spines. Automatic 3-D reconstruction of neurons from light microscopy images, followed by the identification, classification and visualization of dendritic spines is therefore essential for studying neuronal physiology and biophysical properties. In this paper, we present a method to reconstruct dendrites using a surface representation of the dendrite. The 1-D skeleton of the dendritic surface is then extracted by a medial geodesic function that is robust and topologically correct. This is followed by a Bayesian identification and classification of the spines. The dendrite and spines are visualized in a manner that displays the spines' types and the inherent uncertainty in identification and classification. We also describe a user study conducted to validate the accuracy of the classification and the efficacy of the visualization.